ANIMAL EXTRACTION GELATIN LEAVES SKIN

Thus, the collagen content of buffalo hide is higher than cowhide.īuffalo hide collagen exhibits a high hydroxyproline content, which adds complexity to the collagen structure and has been associated with the high gel strength and thermal stability of the produced gelatin ( Giménez et al., 2005 Shoulders and Raines, 2009). Generally, buffalo hide is thicker (6-8 mm) and stronger than that of other animals, accounting for 11.5% of the total body weight, whereas the corresponding value for cowhide is only 9.0% ( Huda et al., 2011 Spanghero et al., 2004). Buffaloes are a representative tropical livestock which provide by-products that are well suited for gelatin manufacture. Gelatin is produced by the partial hydrolysis of the collagen contained in animal raw materials, e.g., buffalo hide ( Zarai et al., 2012). In general, the physicochemical properties of the gelatin complied with the industry standard set by the Gelatin Manufacturers Institute of America, revealing that buffalo hide could serve as a potential alternative source of gelatin.

ANIMAL EXTRACTION GELATIN LEAVES SKIN

The color and Fourier-transform infrared spectrum of the gelatin extracted using 0.9 M HCl were similar to those of commercial bovine skin gelatin. All the gelatins contained α- and β-chain components and several degraded peptides (24-66 kDa). The gel strength did not differ significantly ( p>0.05) according to acid type (280.26-259.62 g Bloom), and the highest viscosity was obtained from the 0.6 M citric acid pretreatment. The highest yield (29.17%) was obtained from pretreatment with 0.9 M HCl. A completely randomized design and the least significant difference test were used in the experimental design, and all measurements were performed in triplicate. Hides were extracted with hydrochloric, citric, and acetic acids at concentrations of 0.3, 0.6, 0.9, 1.2, and 1.5 M. This study aimed to determine the yield and physicochemical properties of gelatins extracted from buffalo hides pretreated with different acids.

Generally, to achieve optimum extraction efficiency, strong acids may be used at a lower acid concentration compared to weak acids.

The acid pretreatment of collagen molecules disrupts their crosslinks and assists in the release of acid-soluble proteins, fats, and other components.

Animal extraction gelatin leaves